Leukotriene B4 (LTB4) kit is so inexpensive - Huaqiang Electronic Network

Kaixin micro test

Test - lowercase jpg

Test probe PH-5H

EL-C1600N100013-B

Leukotriene B4 (LTB4) kit

Introduction

Leukotriene B4 (English: Leukotriene B4) is a leukotriene that is involved in the inflammatory response. It is produced by white blood cells that respond to inflammatory mediators, allowing leukocytes to activate and attach to the endothelium, allowing them to pass through the tissue. It is also a potent chemical inducer in neutrophils and is capable of inducing the formation of reactive oxygen species and the release of enzymes in lysosomes. It is derived from the hydrolysis of leukotriene A4 by leukotriene A4 hydrolase.

Leukotriene B4 (LTB4) kit

Introduction to inspection technology

Double antibody sandwich method

1. Coat: The antibody was diluted to a protein content of 1 to 10 μg/ml with 0.05 MPH 9. 牰 carbonate coating buffer. 0.1 ml was added to the reaction well of each polystyrene plate at 4 ° C overnight. The next day, the solution in the well was discarded and washed 3 times with washing buffer for 3 minutes each time. (referred to as washing, the same below).

2. Loading: Add 0.1 ml of the sample to be tested to the above-mentioned coated reaction well, and incubate at 37 ° C for 1 hour. Then wash. (Do blank holes, negative control wells and positive control wells at the same time).

3. Add enzyme-labeled antibody: Add 0.1 ml of freshly diluted enzyme-labeled antibody (diluted titration) to each well. Incubate at 37 ° C for 0.5 to 1 hour and wash.

4. Add substrate solution to develop color: Add 0.1 ml of temporarily prepared TMB substrate solution to each reaction well at 37 ° C for 10 to 30 minutes.

5. Stop the reaction: Add 0.05 ml of 2M sulfuric acid to each reaction well.

6. Judgment of results: The results can be observed directly on the white background with the naked eye: the darker the color in the reaction well, the stronger the positive degree, the negative reaction is colorless or very light, according to the depth of the color, with "+", The "-" sign indicates. The OD value can also be measured: on the ELISA detector, at 450 nm (if the color is developed by ABTS, 410 nm), the OD value of each well is measured after zero adjustment of the blank control well, if it is greater than 2.1 times the OD value of the specified negative control. Is positive.

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